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KMID : 0545120180280121982
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 12 p.1982 ~ p.1991
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Ethanol induces autophagy regulated by mitochondrial ROS in Saccharomyces cerevisiae
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Jing Hongjuan
Liu Huanhuan
Zhang Lu
Gao Jie
Song Haoran
Tan Xiaorong
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Abstract
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Ethanol accumulation inhibited the growth of Saccharomyces cerevisiae during wine fermentation. Autophagy and the release of reactive oxygen species (ROSs) were also induced under ethanol stress. However, the relation between autophagy and ethanol stress was still unclear. In the study, expression of autophagy genes ATG1 and ATG8 and production of ROS under ethanol treatment in yeast were measured. The results showed that ethanol stress very significantly induced expression of ATG1 and ATG8 genes and the production of peroxide hydrogen (H2O2) and superoxide anion (O2·-). Moreover, the atg1 and atg8 mutants aggregated more H2O2 and O2·- than the wild type yeast. In addition, inhibitors of the ROS scavenging enzyme induced expression of ATG1 and ATG8 genes through increasing the levels of H2O2 and O2·-. Oppositely, glutathione (GSH) and N-acetylcystine (NAC) decreased the ATG1 and ATG8 expression by reducing H2O2 and O2·- production. Besides that, rapamycin and 3-methyladenine caused an obvious change in autophagy levels and simultaneously altered the release of H2O2 and O2·-. At last, inhibitors of mitochondrial electron transport chain (mtETC) increased the production of H2O2 and O2·- and also promoted expression levels of the ATG1 and ATG8 genes. In conclusion, ethanol stress induced autophagy which was regulated by H2O2 and O2·- derived from mtETC. In turn, the autophagy contributed to the elimination H2O2 and O2·-.
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KEYWORD
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Autophagy, ethanol stress, fermentation, hydrogen peroxide, superoxide anion, reactive oxygen specie
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